The proliferation of Lovo cells was quantified by MTT assay.

采用细胞划痕实验检测细胞迁移能力；

BTW significantly inhibited the growth of LOVO cells in dose - dependent manner by flow cytometric analysis.

FCM结果表明,BTW对LOVO细胞有较强的抑制作用,并存在着剂量依赖关系.

Methods:The mice were immunize with LOVO, and the mAb were screened by indirect cell ELISA and immunohistochemistry.

方法：用人结肠癌细胞株LOVO免疫小鼠，用间接细胞ELISA和免疫组化的方法筛选抗结肠癌相关抗原的单抗。