Aim: To evaluate the clinical value of MSP (methylation specific PCR) in detection of tumor suppressor genes methylation in the colorectal cancer.

目的：评价特异性甲基化pcr (msp)分析法检测抑癌基因高甲基化对结、直肠癌的诊疗价值。

Methods a methylation-specific PCR was performed to detect the promoter methylation of RASSF1A gene in 48 tumor tissues, 48 corresponding normal tissues and 48 normal blood plasma.

方法：运用甲基化特异性P CR，检测RASSF1A基因在48例喉癌组织、相对应癌旁组织及48例正常人外周血中的启动子区甲基化情况。

Objective To establish the methylation-specific PCR(MSP) to detect methylation of GTPase regulator associated with the focal adhesion kinase(GRAF) gene promoter.

目的建立甲基化特异性PCR（MSP）技术检测黏着斑激酶相关性鸟苷三磷酸酶调节因子（GRAF）基因启动子甲基化。