Methods Double labeling immunocytochemical staining technique was used.

方法采用免疫细胞化学双重标记技术.

Methods Flow cytometry and immunofluorescence staining technique were used.

方法：采用细胞免疫荧光染色技术和流式细胞术。

Methods: The double labeling immunocytochemical staining technique was used.

方法：免疫细胞化学双重标记技术。

Method Immunocytochemical staining technique by using specific antibody against P2X4 receptor.

方法P2X4受体特异性抗体的免疫细胞化学染色。

Methods A total of 29 human non-Hodgkin′s lymphoma were examined for mutations in exon 2 by using PCR-SSCP silver staining technique.

方法采用聚合酶链反应-单链构象多态银染技术（PCR-SSCP）检测29例非何杰金淋巴瘤患者P16基因的变化。