Secondly, type of elution was considered and type of isocratic elution was attempted firstly.

第二是洗脱方式，可先用等度洗脱方式进行尝试。

Synthetic peptide purification. Are isocratic elution and volume overload options to improve process economics?

合成蛋白提纯-无梯度洗脱和容量超负荷的取舍能提高工艺经济性？

Diastereomers of cinchonine and cinchonidine were fully separated both under isocratic and gradient elutions on the chiral monolithic column.

在等度及梯度洗脱条件下，非对映异构体辛可宁与辛可尼丁被完全分离。